Urinary Tract Infections & URINE CULTURE

Here are the slides, plates, test tubes and apparatus from our first microbiology practical class of semester 6.

Slides :


1. Gram -ve bacilli

2. Streptococci in chain

3. Gram +ve cocci arranged in grape-like clusters

    - STAPHYLOCOCCI

4. Acid fast bacilli - MYCOBACTERIUM TUBERCULOSIS

stain - Ziehl Neelsen

bacilli - pink

pus - blue

Plates :

1. Non-lactose fermenting colonies on MacConkey's agar

2. Pigmentation of Pseudomonas on NA

3. Swarming of Proteus on blood agar

4. E.coli on MacConkey's agar

      

5. Staphylococci on NA

6. Miles & Misra

    - to count the colonies and calculate the number of organisms per ml of       urine

    - more accurate than dip slide

    - quantitative

Test tubes :

1. Egg saline

    - enriched media for TB

2. Lowenstein Jensen medium

    - selective media for TB

Apparatus :

1. Dip slide

    - to count the colonies and calculate the number of organisms per ml of       urine

    - semiquantitative

2. Sterile container

    - to collect mid-stream urine sample