Lab Diagnosis of Gonorrhea and some pic

Second microbiology practical class of semester 6.

SLIDES :

1. Gram -ve diplococci : NEISSERIA culture

2. Gram -ve diplococci : NEISSERIA smear

     stain - Gram stain

3. Gram -ve diplococci : NEISSERIA smear

     stain - Methylene blue

PLATES :

1. Blood agar

2. Chocolate agar

3. Thayer-Martin

    - chocolate agar + VCN (vancomycin, colistin, nystatin)

    - vancomycin for Gram +ve bacteria, colistin for Gram -ve bacteria, nystatin for fungi

4. Oxidase test

-Violet colonies

- IMPORTANT CHARACTER distinguish from meningococcus is ability producing acid from glucose not maltose

APPARATUS :

Candle jar

-we can also use gas pack jar.

PHOTOS :

1. Chlamydia - Giemsa stain

-Obligate intracellular 

-not affected by B-lactam antibiotics(absense of peptidoglycan layer in cell wall)

-sensitive to sulphonamides.

2. Chlamydia - McCoy's cells

Lab diagnosis:

-cytology 

-isolation

-direct antigen detection(IF,ELISA)

-NA probes

-serology

3. Mycoplasma - phleomorphic appearance

-SLOW GROWERS: incubated at least 48-96 hrs but maybe several days or even weeks before growth appears..