Anaerobiosis - Spore stain

Here are the slides and photos from our first microbiology practical class.

Spore bearer bacilli - GRAM STAIN

protoplasm - deeply coloured/violet
spore - unstained/clear area

Spore bearer bacilli - MODIFIED ZIEHL-NEELSEN STAIN

protoplasm - blue

spore - bright red/pink 

Spore bearer bacilli - NEGROSIN FUCHSIN STAIN

protoplasm - unstained

spore - red/pink

DARK BACKGROUND

ANAEROBIC JAR

COOKED MEAT MEDIUM (ROBERTSON'S MEDIUM)

Reducing substances arise from sterilized muscle tissue which are :

• blood pigments of the meat
• unsaturated fatty acids

DEEP NUTRIENT AGAR