Practical Exam Getting Nearer..Be prepared..GUDLUCK..!

Macriconidia of Microsporium Canis

How many species of Microsporum?

Other type of microsporum 

 ASPERGILLUS NIGER

Much colour of NIGER..opss..BLACK DOT under microscope..

Very common disease in Egypt causing OTITIS EXTERNA.?

Predisposing factor? 

ASPERGILLUS FUMIGATUS 

Under microscope..we see GREEN rather than BLACK..

Main disease cause by this organism?

Predisposing factor? 

  

 GRAY PATCH

Tinea Capitis (Ectothrix)

 Describe the lesion.

ONYCHOMYCOSIS

Describe the treatment. 

  

BLACK DOT

Tinea Capitis (Endothrix)

Describe the lesion.

  

FAVUS

What cause the permanent loss of hair?  

 What is formed on the head?

 CANDIDA ALBICANS IN TEST TUBE

In RAT (Rice Agar Tween) test, incubation in what degree for how long?

How to detect presence of this organism in the test?

THRUSH STOMATITIS

Seen in what type of patients? 

TINEA CORPORIS 

 How can we collect this specimen?

Can we use cycloheximide in culturing it?

CANDIDA on plate.

Why cant we use cycloheximide culturing it?

Name 2 stains for histopatological diagnosis.

ANY LACK OF INFORMATION?

PLEASE FORGIVE MYSELF..:p

THANK YOU

FORMAT for MICROBIOLOGY Practical Exam

• 6-8 spots
• Identify & answer questions
• For each spot we may have test tube, microscope, plate, photo or other apparatus
• For slides, we have 13 :
• Spore bearer bacilli - GRAM STAIN
• Spore bearer bacilli - MODIFIED ZIEHL-NEELSEN STAIN
• Spore bearer bacilli - NEGROSIN FUCHSIN STAIN
• Gram +ve diplococci : PNEUMOCOCCI
• Acid fast bacilli : MYCOBACTERIUM TUBERCULOSIS
• Gram -ve bacilli
• Gram -ve diplococci : NEISSERIA smear (Methylene blue)
• Gram -ve diplococci : NEISSERIA smear (Gram stain)
• Gram -ve diplococci : NEISSERIA culture
• Candida
• Aspergillus nigar
• Aspergillus fumigatus
• Macroconidia of Microsporum canis
• In the exam, slides can be in the form of photo also
• For photos, we have 8 :
• Candida culture in test tube
• Thrush stomatitis
• Endothrix (black dot)
• Ectothrix (gray patch)
• Ectothrix (cotton blue)
• Tinea capitis (favus)
• Tinea corporis
• Onychomycosis
• For plates, we have 5 :
• Blood agar
• Chocolate agar
• Candida
• Aspergillus nigar
• Aspergillus fumigatus
• For test tubes, we have 4 :
• Cooked meat medium
• Deep nutrient agar
• Lowenstein Jensen Medium (LJ)
• Egg saline

GOOD LUCK!! :)

Lab Diagnosis of FUNGAL INFECTION

These are slides, photos and plates from our 3rd microbiology practical class.


Slides


Candida

Aspergillus nigar

 

Aspergillus fumigatus

Macroconidia of Microsporum canis

Photos

Candida culture in test tube

Thrush stomatitis

Endothrix (black dot)

Ectothrix (gray patch)

Ectothrix (cotton blue)

Tinea capitis (favus)

Tinea corporis

Onychomycosis

Plates

Candida

Aspergillus nigar

Aspergillus fumigatus

-PHARMA- VIDEO : ANAESTHESIA experiment

We can see the stages of general anaesthesia when using ether, halothane and isoflurane

PNEUMOCYSTIS JIROVECI

Causative agents of MENINGITIS

Here are the slides, test tubes and plates from our second microbiology practical class.

Gram +ve diplococci : PNEUMOCOCCI

Acid fast bacilli : MYCOBACTERIUM TUBERCULOSIS

stain - Ziehl Neelsen stain

bacilli - pink

pus - blue

Gram -ve bacilli

Gram -ve diplococci : NEISSERIA smear

stain - Methylene blue

Gram -ve diplococci : NEISSERIA smear

stain - Gram stain

Gram -ve diplococci : NEISSERIA culture

Test tubes :

1. Lowenstein Jensen Medium (LJ)

2. Egg saline

Plates :

1. Blood agar

2. Chocolate agar