Lab Diagnosis of Gonorrhea and some pic

Second microbiology practical class of semester 6.

SLIDES :

1. Gram -ve diplococci : NEISSERIA culture

2. Gram -ve diplococci : NEISSERIA smear

     stain - Gram stain

3. Gram -ve diplococci : NEISSERIA smear

     stain - Methylene blue

PLATES :

1. Blood agar

2. Chocolate agar

3. Thayer-Martin

    - chocolate agar + VCN (vancomycin, colistin, nystatin)

    - vancomycin for Gram +ve bacteria, colistin for Gram -ve bacteria, nystatin for fungi

4. Oxidase test

-Violet colonies

- IMPORTANT CHARACTER distinguish from meningococcus is ability producing acid from glucose not maltose

APPARATUS :

Candle jar

-we can also use gas pack jar.

PHOTOS :

1. Chlamydia - Giemsa stain

-Obligate intracellular 

-not affected by B-lactam antibiotics(absense of peptidoglycan layer in cell wall)

-sensitive to sulphonamides.

2. Chlamydia - McCoy's cells

Lab diagnosis:

-cytology 

-isolation

-direct antigen detection(IF,ELISA)

-NA probes

-serology

3. Mycoplasma - phleomorphic appearance

-SLOW GROWERS: incubated at least 48-96 hrs but maybe several days or even weeks before growth appears..

Urinary Tract Infections & URINE CULTURE

Here are the slides, plates, test tubes and apparatus from our first microbiology practical class of semester 6.

Slides :


1. Gram -ve bacilli

2. Streptococci in chain

3. Gram +ve cocci arranged in grape-like clusters

    - STAPHYLOCOCCI

4. Acid fast bacilli - MYCOBACTERIUM TUBERCULOSIS

stain - Ziehl Neelsen

bacilli - pink

pus - blue

Plates :

1. Non-lactose fermenting colonies on MacConkey's agar

2. Pigmentation of Pseudomonas on NA

3. Swarming of Proteus on blood agar

4. E.coli on MacConkey's agar

      

5. Staphylococci on NA

6. Miles & Misra

    - to count the colonies and calculate the number of organisms per ml of       urine

    - more accurate than dip slide

    - quantitative

Test tubes :

1. Egg saline

    - enriched media for TB

2. Lowenstein Jensen medium

    - selective media for TB

Apparatus :

1. Dip slide

    - to count the colonies and calculate the number of organisms per ml of       urine

    - semiquantitative

2. Sterile container

    - to collect mid-stream urine sample

ATTENTION!!!...OUR LAST SEMESTER FOR THEORETICAL YEAR...:)

WELCOME BACK..to 'new' Egypt. Why is it new? Don't know lah..:P 
By the way..i just want to say that our 'war' is still not finish yet.."soldiers,be prepared" 
In this semester don't ever forget that we will be presenting our assignment on HUMAN RIGHT which will begin next week InsyaAllah..So hopefully that we stay tune with the channel,..opss sorry,stay alert with your own presentation so that make the professor easy close the eyes and write full marks..:) InsyaAllah..


And don't forget to be prepared yourself to be present to practical classes which will be set up next week in which only physiology class begin..Our GS3 leader said that he would like to show to the other section that this semester will have no absences in practical classes...unless unexcused reason..:P 


Hope that all of us will gain new spirit of studying..FI SABILILLAH..never give up..No fails.. No one would be left behind InsyaAllah..:) 

 

-MICROBIOLOGY- Exam Review

-MICROBIOLOGY- Answer Scheme

The questions for both years are EXACTLY the same.

-PHARMA- Answer Scheme

In this document, the words written in red colour are not included in our batch's syllabus for this semester. Those highlighted in green have been asked for 2 years in a row.

-PHARMA- Exam Review